Tb-doped BSA-gold nanoclusters as a bimodal probe for the selective detection of TNT.

Trinitrotoluene (TNT) is a widely used explosive belonging to the family of nitroaromatic compounds, and its misuse poses a significant threat to society. Herein, we propose a Tb-BSA-AuNC fluorescent and colorimetric sensing probe for the selective onsite detection of TNT in the aqueous phase. Tb-doped BSA-protected gold nanoclusters (Tb-BSA-AuNCs) were synthesized by a microwave-assisted method, and TNT detection was carried out utilizing the chemistry of Meisenheimer complex formation.
Tb doping of gold nanoclusters was demonstrated to facilitate better electron shuttling effects and thereby improve the efficiency of complex formation between the TNT and gold nanoclusters. A paper strip assay was also developed for TNT detection with the designed probe. Limits of detection and quantification of 0.2136 mM and 0.7120 mM, respectively, were achieved. Graphical abstract.

Exploring the interactions of a Tb(III)-quercetin complex with serum albumins (HSA and BSA): spectroscopic and molecular docking studies.

  • Serum albumins (human serum albumin (HSA) and bovine serum albumin (BSA), two main circulatory proteins), are globular and monomeric macromolecules in plasma that transport many drugs and compounds. In the present study, we investigated the interactions of the Tb(III)-quercetin (Tb-QUE) complex with HSA and BSA using common spectroscopic techniques and a molecular docking study.
  • Fluorescence data revealed that the inherent fluorescence emission of HSA and BSA was markedly quenched by the Tb-QUE complex through a static quenching mechanism, confirming stable complex formation (a ground-state association) between albumins and Tb-QUE. Binding and thermodynamic parameters were obtained from the fluorescence spectra and the related equations at different temperatures under biological conditions. The binding constants (Kb ) were calculated to be 0.8547 × 103 M-1 for HSA and 0.1363 × 103 M-1 for BSA at 298 K. Also, the number of binding sites (n) of the HSA/BSA-Tb-QUE systems was obtained to be approximately 1. Thermodynamic data calculations along with molecular docking results indicated that electrostatic interactions have a main role in the binding process of the Tb-QUE complex with HSA/BSA.
  • Furthermore, molecular docking outputs revealed that the Tb-QUE complex has high affinity to bind to subdomain IIA of HSA and BSA. Binding distances (r) between HSA-Tb-QUE and BSA-Tb-QUE systems were also calculated using the Forster (fluorescence resonance energy transfer) method. It is expected that this study will provide a pathway for designing new compounds with multiple beneficial effects on human health from the phenolic compounds family such as the Tb-QUE complex.

BSA-AuNPs@Tb-AMP metal-organic frameworks for ratiometric fluorescence detection of DPA and Hg2.

An easy and effective strategy for synthesizing a ratiometric fluorescent nanosensor has been demonstrated in this work. Novel fluorescent [email protected] (BSA, bovine serum albumin; AMP, adenosine 5′-monophosphate; AuNPs, Au nanoparticles) metal-organic framework (MOF) nanostructures were synthesized by encapsulating BSA-AuNPs into Tb-AMP MOFs for the detection of 2,6-pyridinedicarboxylic acid (DPA) and Hg2+ . DPA could strongly co-ordinate with Tb3+ to replace water molecules from the Tb3+ center and accordingly enhanced the fluorescence of Tb-AMP MOFs. The fluorescence of BSA-AuNPs at 405 nm remained constant.
While the fluorescence of BSA-AuNPs at 635 nm was quenched after Hg2+ was added, the fluorescence of Tb-AMP MOFs remained constant. Accordingly, a ratiometric fluorescence nanosensor was constructed for detection of DPA and Hg2+ . The ratiometric nanosensor exhibited good selectivity to DPA over other substances. The F545 /F405 linearly increased with increase of DPA concentration in the range of 50 nM to 10 μM with a detection limit as low as 17.4 nM. F635 /F405 increased linearly with increase of Hg2+ concentration ranging from 50 nM to 1 μM with a detection limit as low as 20.9 nM. Additionally, the nanosensor could be successfully applied for the determination of DPA and Hg2+ in running water.

Ratiometric fluorescence detection of superoxide anion based on AuNPs-BSA@Tb/GMP nanoscale coordination polymers.

A novel ratiometric fluorescence nanosensor for superoxide anion (O2•- ) detection was designed with gold nanoparticles-bovine serum albumin (AuNPs-BSA)@terbium/guanosine monophosphate disodium (Tb/GMP) nanoscale coordination polymers (NCPs) ([email protected]/GMP NCPs). The abundant hydroxyl and amino groups of AuNPs-BSA acted as binding points for the self-assembly of Tb3+ and GMP to form core-shell [email protected]/GMP NCP nanosensors.
The obtained probe exhibited the characteristic fluorescence emission of both AuNPs-BSA and Tb/GMP NCPs. The AuNPs-BSA not only acted as a template to accelerate the growth of Tb/GMP NCPs, but also could be used as the reference fluorescence for the detection of O2•- . The resulting [email protected]/GMP NCP ratiometric fluorescence nanosensor for the detection of O2•- demonstrated high sensitivity and selectivity with a wide linear response range (14 nM-10 μM) and a low detection limit (4.7 nM).

Spectroscopic investigation on sonodynamic and sonocatalytic damage of BSA molecules by Thymol Blue (TB) derivants under ultrasonic irradiation.

In this paper, the Thymol Blue derivants including Thymol Blue (thymolsulfonphthalein), Thymol Blue-DA (3,3′-Bis [N,N-bis (carboxymethyl) aminomethyl] thymolsulfonphthalein) and Thymol Blue-DA-Fe(III) (3,3′-Bis [N,N-bis (carboxymethyl) aminomethyl] thymolsulfonphthalein-Ferrous(III)) were adopted as sonosensitizers to study the sonodynamic and sonocatalytic activities under ultrasonic irradiation. At first, the interaction of Thymol Blue derivants with bovine serum albumin (BSA) was studied by fluorescence spectroscopy. On that basis, the sonodynamic and sonocatalytic damages of Thymol Blue derivants to BSA under ultrasonic irradiation were investigated by the combination of UV-vis, circular dichroism (CD) and fluorescence spectroscopy.
Meanwhile, some influenced factors (ultrasonic irradiation time, Thymol Blue derivants concentration and ionic strength) on the damaging degree of BSA molecules were also reviewed. In addition, synchronous fluorescence spectra were used to estimate the binding and damage sites of Thymol Blue derivants to BSA. Finally, the generation of ROS during sonodynamic and sonocatalytic processes was confirmed by the method of Oxidation-Extraction Spectrometry (OEP). Perhaps, this paper may offer some important subjects for the study of Thymol Blue derivants in sonodynamic therapy (SDT) and sonocatalytic therapy (SCT) technologies for tumor treatment and the effect of the amino acid and central metal.

TBS blocking buffer with BSA

SD8113 Bio Basic 1pk, 1L 100.72 EUR


I004 Cygnus Technologies 1000 ml 262.8 EUR

TBS Buffer, 10X

T8057-100 GenDepot 1L 138 EUR

TBS Buffer, 10X

T8057-105 GenDepot 5x1L 343.2 EUR

TBS, 20X pH 7.4

18-236 Genesee Scientific 500ml/Unit 303 EUR

TBS, 20X pH 7.4

18-236B Genesee Scientific 1000ml/Unit 342 EUR

TBS Wash Buffer Pack

AR0144 BosterBio 500mL/pack 72 EUR

TBS Blocking Buffer Pack

AR0143 BosterBio 200mL/pack 78 EUR

TBS Wash Concentrate, 20X

F004 Cygnus Technologies 50 ml 216 EUR

TBS Wash Concentrate, 20X

F004-1000 Cygnus Technologies 1000 ml 483.6 EUR

EZBlock? (TBS) Blocking Buffer

2117-1000 Biovision each 254.4 EUR

EZBlock? (TBS) Blocking Buffer

2117-200 Biovision each 150 EUR

Blotto 2 buffer (1% milk, 1% BSA, 0.05% tween in TBS buffer for phosphoproteins) for Western

80403-500 Alpha Diagnostics 500 ml 92.4 EUR

TBS Buffer, 20X pH 7.4

T8054-050 GenDepot 500ml 103.2 EUR

TBS Buffer, 20X pH 7.4

T8054-101 GenDepot 1L 118.8 EUR

TBS Buffer, 20X pH 7.6

T8055-050 GenDepot 500ml 103.2 EUR

TBS Buffer, 20X pH 7.6

T8055-101 GenDepot 1L 118.8 EUR

TBST (TBS-Tween 20), 10XpH 7.6

UA0046 Bio Basic 500ml 83.92 EUR

TBS Buffer, 10X , Refill Pack

T8057-401 GenDepot 1 Gal 218.4 EUR

EZBlock? T20 (TBS) Blocking Buffer

2140-1000 Biovision each 261.6 EUR

EZBlock? T20 (TBS) Blocking Buffer

2140-200 Biovision each 144 EUR

Tris Buffered Saline (TBS) Powder

AR0031 BosterBio 2000mL/pack 63.6 EUR

Strps o 8PCR tbs PP attch transp sngl cps 0.15ml l-prfl wht tbs

MOL2272 Scientific Laboratory Supplies PK120 216 EUR

Accuris QuickSilver TBS Buffer Powder

EB1202 Scientific Laboratory Supplies PK100 278.4 EUR

Strps o 8PCR tbs w attch ind flt cps 3rdgs btwn tbs 0.15mL l-prfl

MOL2274 Scientific Laboratory Supplies PK120 205.2 EUR

TBST (TBS-Tween 20), 10X pH 7.6

18-235 Genesee Scientific 500ml/Unit 300 EUR

TBST (TBS-Tween 20), 10X pH 7.6

18-235B Genesee Scientific 1000ml/Unit 334 EUR

TBS with non fat milk premix

SD8104 Bio Basic 1pk 76.19 EUR

1L TBS (10X) Tris Buffered Saline

NAT1244 Scientific Laboratory Supplies 1L 99.6 EUR

Strps o 8PCR tbs w attch ind flt cps 3 rdgs btwn tbs 0.2mL std prfl

MOL2270 Scientific Laboratory Supplies PK120 205.2 EUR

[Luminescence properties of rare earth complexes Tb(BSA)4].

A new rare earth complex Tb(BSA)4 was synthesized and studied. Pure green and narrow band emission was generated from the device with structure ITO/PVK:Tb(BSA)4 /Alq3 /LiF/Al, where PVK was used to improve the film-formation and hole-transport property of the Tb(BSA)4.
The absorption mechanism, and the photoluminescence and electroluminescence mechanisms are discussed. It has been proved that there exists energy transfer from PVK to Tb(BSA)4 and the mechanisms of photoluminescence and electroluminescence are different. The effect of different ratios of PVK on the device characteristics is also studied.

Leave a Reply

Your email address will not be published.