HTRF: a technology tailored for biomarker determination-novel analytical detection system suitable for detection of specific autoimmune antibodies as biomarkers in nanogram level in different body fluids.
BACKGROUNDClassical methods of gene product analysis such as binding assays (e.g., ELISA, protein chip technology) are usually time-consuming, lab-intensive, a lot much less delicate, and lack high-throughput functionality.
In addition, all present methods used to measure proteins necessitate a quantity of divisions of the distinctive sample and specific particular person assessments carried out for each substance, with an associated worth for each take a take a look at.
METHODSTogether with a small biotech agency, we developed a new and fashionable analytical detection system primarily based totally on homogenous time-resolved fluorescence (HTRF) technology.
Our system facilitates the occasion of immune assays that measure selective different analytes such as chosen biomarkers in a small sample amount at decrease than 20 min with a a lot better sensitivity in comparability with frequent binding assay applications such as enzyme-linked immunosorbent assay (ELISA).
Recent advances of the equipment of this novel detection system combine the power of miniaturization, microfluidics, larger linear range, and faster quantification.
HTRF: a technology tailored for biomarker determination-novel analytical detection system suitable for detection of specific autoimmune antibodies as biomarkers in nanogram level in different body fluids.
RESULTSThe vitality of the HTRF technology offers good promise for point-of-care scientific testing and monitoring of many crucial analytes such as disease-specific biomarkers in the nanogram level in different human body fluids such as CSF, blood, serum, plasma, and saliva. The linear dynamical range of our HTRF assay was determined between 2.5 and 100 ng/mL.
Human Pancreatic carcinoma markers-CA242 ELISA Kit
Description: A sandwich ELISA kit for quantitative measurement of Human CA242 (Pancreatic Carcinoma Markers-CA242) in samples from Serum, Plasma, Cell supernatant
Description: IC50: 7 nMNeutrophil Elastase Inhibitor is a neutrophil elastase inhibitor.Human neutrophil elastase (HNE) is a member of the chymotrypsin superfamily of serine proteases that is involved in the response to inflammatory stimuli.
Description: IC50: 7 nMNeutrophil Elastase Inhibitor is a neutrophil elastase inhibitor.Human neutrophil elastase (HNE) is a member of the chymotrypsin superfamily of serine proteases that is involved in the response to inflammatory stimuli.
Description: IC50: 7 nMNeutrophil Elastase Inhibitor is a neutrophil elastase inhibitor.Human neutrophil elastase (HNE) is a member of the chymotrypsin superfamily of serine proteases that is involved in the response to inflammatory stimuli.
Description: IC50: 7 nMNeutrophil Elastase Inhibitor is a neutrophil elastase inhibitor.Human neutrophil elastase (HNE) is a member of the chymotrypsin superfamily of serine proteases that is involved in the response to inflammatory stimuli.
Description: Elastase is a serine protease that breaks down elastin and is produced in the pancreas. Elastase is produced as an inactive proelastase and activated by trypsin in the duodenum. Elastase plays an important role in inflammation.
Description: A polyclonal antibody raised in Sheep that recognizes and binds to Human HNE / Neutrophil Elastase . This antibody is tested and proven to work in the following applications:
Description: A competitive ELISA for quantitative measurement of Rat Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Neutrophil Elastase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Precision and accuracy calculated for inter- as successfully as intra-assays was decrease than ± 10 %. Intra-assay and inter-assay precision for extreme, medium, and low analyte concentrations current indicate CV values decrease than ± 10 %.
Intra- and inter-assay accuracy for all three concentrations current indicate restoration values of 80-120 %.
CONCLUSIONSThe purpose of this work is to clarify the occasion and establishment of this novel HTRF system that permits the very fast detection and quantification of biomarkers in different human body fluids. Furthermore, a specific antibody combination that assures a specific binding of the correct refolded autoimmune IgG is evaluated.
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